Composite
Part:BBa_K4164008
Designed by: Bo Cheng Group: iGEM22_NAU-CHINA (2022-09-30)
constitutive expression of BSS
In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried low intensity promoter at first.This composite part contains the low intensity promoter (BBa_J23105) and RBS. The composite part can be directly imported into plasmid and express BSS. If you want to learn about the detailed introduction of BSS, you can click the link below. https://parts.igem.org/Part:BBa_K4164001
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 965
Illegal NgoMIV site found at 1016 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |